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Cartridge Based Nucleic Acid Amplification Test [CBNAAT]
Learning Objectives- Provide an overview of the CBNAAT- Fully automated (GeneXpert) platform and that it uses RTPCR as the base molecular technology. Describe the name CBNAAT and discuss the use of the word cartridge.
- Discuss the important advantages of the platform.
- Rapid test with high reliability
- Automated test
- The type of cartridge would determine the test/ assay that would be performed.
- Discuss the parts of a CBNAAT machine and the various configurations it comes in.
- Mention that they can identify the nearest CBNAAT laboratory from Nikshay Directory or the Nikshay Arogya Saathi application
Cartridge Based Nucleic Acid Amplification Test (CBNAAT) is a rapid molecular diagnostic test. It is used for diagnosis of Tuberculosis (TB) and Rif-resistant Tuberculosis (RR-TB) in NTEP. Results are obtained from unprocessed sputum samples in about 2hours which helps in early detection and treatment of TB patients.
India has vast number of CBNAAT laboratories which are utilized for TB/RR-TB detection and Universal Drug Susceptibility Testing (UDST) under the National TB Elimination Program (NTEP).
Figure: CBNAAT Cartridge and Machine in Use (Image courtesy: USAID supported Challenge TB Project)
The CB-NAAT system detects DNA sequences specific for Mycobacterium tuberculosis complex and rifampicin resistance by Polymerase Chain Reaction (PCR). It concentrates Mycobacterium tuberculosis bacilli from sputum samples, isolates genomic material from the captured bacteria by sonication and subsequently amplifies the genomic DNA by PCR. The process identifies clinically relevant rifampicin resistance-inducing mutations in the RNA polymerase beta (rpoB) gene in the Mycobacterium tuberculosis genome in a real-time format using fluorescent probes called molecular beacons.
Video: Cartridge-Based Nucleic Acid Amplification Test [CBNAAT] - GeneXpert Technology
Resources
- Training Module (1-4) for Program Managers and Medical Officers, NTEP, MoHFW, 2020.
- India TB Report 2021, National TB Elimination Program (NTEP), MoHFW, 2021.
Assessment Questions
|
Question |
Answer 1 |
Answer 2 |
Answer3 |
Answer 4 |
Correct Answer |
Correct explanation |
Part of pre-test |
Part of post-test |
|
Under NTEP, CBNAAT is offered upfront for which of these categories? |
PLHIV |
Paediatric presumptive TB |
Presumptive DR-TB |
All of the above |
4 |
Under NTEP, CBNAAT is recommended upfront for People living with HIV, Paediatric Presumptive TB patients, Presumptive DR-TB patients and patients notified from the Private sector. |
Yes
|
Yes |
|
CBNAAT requires the processing of sputum samples before testing |
True |
False |
|
|
2 |
Results are obtained from unprocessed sputum samples in about 2hours from a CBNAAT machine |
Yes
|
Yes |
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