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Prevention of False Positive Results During Smear Microscopy
Learning Objectives- Learn about potential sources of error and factors contributing to false positive results in smear microscopy.
- Implement quality assurance measures, including adherence to standard operating procedures and proficiency testing, in preventing false positive results.
Content
False positive results can be prevented by using the following measures:
- Always use new, unscratched slides.
- Use a separate broomstick for each sample.
- Always use filtered Carbol fuchsin.
- Do not allow the Carbol fuchsin to dry during staining.
- Decolorize adequately with Sulphuric acid.
- Make sure that there are no food particles or fibers in the sputum sample.
- Never allow the oil immersion applicator to touch a slide.
- Label sputum containers, slides and laboratory forms accurately.
- Cross-check the number on the laboratory form and sputum container before recording.
- Record and report the results accurately.
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