Prevention of False Positive Results During Smear Microscopy

Prevention of False Positive Results During Smear Microscopy
Learning Objectives
Learn about potential sources of error and factors contributing to false positive results in smear microscopy.

Implement quality assurance measures, including adherence to standard operating procedures and proficiency testing, in preventing false positive results.

Content

False positive results can be prevented by using the following measures:

Always use new, unscratched slides.
Use a separate broomstick for each sample.
Always use filtered Carbol fuchsin.
Do not allow the Carbol fuchsin to dry during staining.
Decolorize adequately with Sulphuric acid.
Make sure that there are no food particles or fibers in the sputum sample.
Never allow the oil immersion applicator to touch a slide.
Label sputum containers, slides and laboratory forms accurately.
Cross-check the number on the laboratory form and sputum container before recording.
Record and report the results accurately.

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