Truenat DNA Extraction Cartridge

Truenat cartridges are components of Trueprep AUTO v2 Universal Cartridge Based Sample Prep Kit. As shown in the figure below, each cartridge is:

• Used with Trueprep Device for DNA extraction and purification
• Disposable
• Single-use
• Preloaded with Internal Positive Control.

Truenat cartridges are used with Trueprep devices for DNA extraction and purification. The components of the cartridge are illustrated in the following figure and include:

• Sample Chamber - Processed samples (pretreated with lysis buffer) are added to the sample chamber of the cartridge for processing on the Trueprep device.
• Matrix Chamber - DNA released by chemical and thermal lysis cells binds to the proprietary matrix here.
• Elute Chamber - Trapped DNA is washed with buffers to remove Polymerase Chain Reaction (PCR) inhibitors and is eluted from the matrix using the elution buffer. The purified DNA is collected from here.
• Waste generated is contained within the dump region inside the cartridge.

Figure: Inside of a TrueNAT Cartridge; Source: Practical Guide to Implementation of TrueNAT Tests for the Detection of TB and Rifampicin Resistance

Resources

• Truenat MTB Kit Insert.
• Trueprep AUTO Universal Cartridge Based Sample Prep Kit.
• Trueprep AUTO Universal Cartridge Based Sample Prep Device.
• Practical Guide to Implementation of TrueNAT Tests for the Detection of TB and Rifampicin Resistance.