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Indications for retesting in CBNAAT
Learning ObjectivesDiscuss the process to be followed for retest procedure for CBNAAT.
The assay needs to be repeated by using a new cartridge if one of the following test results occur:
INVALID: An INVALID result indicates that Sample Processing Control (SPC) failed. The sample was not properly processed, or Polymerase Chain Reaction (PCR) was inhibited.
ERROR: An ERROR indicates that Probe Check Control (PCC) failed, and the assay was aborted possibly due to the reaction chamber being filled improperly, a reagent probe integrity, syringe pressure issues, or failure of the CBNAAT module.
NO RESULT: A NO RESULT indicates that insufficient data were collected. For example, the operator stopped a test that was in progress.
RIF Indeterminate: RIF indeterminate result indicates that the sample has a less bacterial load.
Program guidelines recommend obtaining a second specimen to confirm rifampicin resistance in these scenarios.
How to Perform the Retest?
- Leftover sputum or fresh sputum or reconstituted sediment:
Treat it with a new Sample Reagent (SR) and load it into the new CBNAAT cartridge.
- Sufficient leftover SR-treated sample:
Use within 4 hours of initial addition of SR to the sample - Load into the new CBNAAT cartridge.
Do not use SR-treated sample if it is more than 4 hours old - Over-treatment may lead to false-positive test results.
Always use a new cartridge!
Video : Retest Protocol for CBNAAT
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