CBNAAT Troubleshooting: Failures without Error Codes

Some Cartridge-based Nucleic Acid Amplification Test (CBNAAT) machine errors will indicate test failure without Error Codes.

Error: Invalid

Problem: 

Sample Processing Control (SPC) failed.

Origin: 

Polymerase Chain Reaction (PCR) was inhibited due to food particles or blood in the sample.

Solution:  

Laboratory Technician (LT) needs to collect another specimen, if necessary.

Prevention: 

• Before mixing with the sample reagent for decontamination, check whether the sample contains food particles or blood. 
• Allow food particles to settle down before adding the sample to the cartridge.

If the problem persists, one needs to contact the manufacturer for technical support.

Figure: Error - Invalid

Error: No Result

Problem:

Test could not be completed and insufficient data collected.

Origins:

Software stops working before the test is completed due to: 

• Windows or software freeze 
• Power failure 
• STOP TEST function was activated (accidentally or deliberately)

Solution:

Secure the power supply, restart the machine and repeat the test with a new cartridge.

Contact an authorized service provider if the problem persists.

Figure: Error - No Result

Video : CBNAAT Troubleshooting: Failures without Error Codes

Resources

• GeneXpert Package Insert