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  • Inoculation of Brain Heart Infusion [BHI] Agar

    Learning Objectives
    • Understand the purpose of inoculating Brain Heart Infusion (BHI) agar in microbiological culture procedures.
    • Learn the techniques to check for contamination in MGIT broth.
    • Learn the techniques to check the sterility of reagents/ processed specimens.
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Brain Heart Infusion (BHI) Agar is a general-purpose medium​ that is:

  • Prepared using dehydrated ingredients
  • Suitable to grow bacteria, yeasts and moulds​
  • ​Used for reagent quality control and contamination check for liquid culture​.

 

To check contamination in Mycobacteria Growth Indicator Tube (MGIT) broth:

  • Visually inspect MGIT tubes for heavy turbidity. Heavy turbidity indicates contamination as Mycobacterium tuberculosis growth appears in form of particles without any significant turbidity.
  • Divide the BHI agar plate into four quadrants, sub-culture loopful of turbid growth from MGIT tubes in each quadrant (small streak for each specimen, properly labelled).
  • Incubate at 37ºC and observe after 24-48 hours for growth of contaminants.

 

To check the sterility of reagents/ processed specimens:

  • Spread one drop of reagent/ sediment on BHI plate​.
  • Incubate the inoculated BHI plates at 37℃ for 24-48 hours to check for contaminants​.

 

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