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General Instructions

 

  • Thoroughly clean the work bench with 1% sodium hypochlorite solution​.
  • Run the wash cycle A and B in GT Blot.​
  • After cleaning, prepare reagents as per the calculation table.
  • Pour prepared reagents into reagent bottles provided with GT Blot​.
  • Place GT Blot reagent bottles back to machine​.
  • Cover the bottles with a tubed lid​.
  • Using clean/ new tweezers, place strips onto a clean sheet of paper and label them same as that in the work sheet​.
  • Use a clean/ new tray, check for any wear/ tear.

 

Steps for hybridization using twincubator​ are shown in the table below.

 

Table: Hybridization Steps using Twincubator

STEP-1

DISPENSE 20ΜL DENATURATION (DEN) SOLUTION IN A CORNER OF EACH WELL​

NOTE: NO NEED TO CHANGE TIPS​

Step-2
  • Add 20µl amplicon to DEN and carefully mix by pipetting up and down​
  • Incubate for 5 min​
  • Change tips for every sample​
  • Carefully place the tray in GT Blot​
  • Close the GT Blot lid​
  • Press start​
  • Select no. of wells (always in even numbers) ​
  • Press start​

Note: Change tips between samples​

Step-3
  • The instrument will automatically dispense hybridization solution to each well selected​
  • Message – ‘Add amplicon’ will be displayed​

Step-4
  • Carefully place numbered strips in each well, up (face-side), using clean/ new plastic tweezers​
  • Close the lid of GT Blot​
  • Press start​
  • All the subsequent steps will be carried out automatically in a fully automated GT Blot machine​

 

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