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Each strip for Second Line - Line Probe Assay (SL - LPA) has a total of 27 reaction zones (Figure below).

Figure: Key Zones of SL - LPA Evaluation Strip; Source: GenoType MTBDRsl VER 2.0 kit, Instructions for Use.

 

 

Evaluation of Assay Strip for SL- LPA Zones:

 

  • Evaluation of Conjugate Control (CC), Amplification Control (AC), M. tuberculosis complex (TUB) are the same as described for First Line - LPA.
  • Locus Controls (gyrA, gyrB, rrs, eis)
    • Detect gene region specific (gyrA/ gyrB/ rrs/ eis)
  • Wild type probes
    • Comprise the resistance regions of respective genes (gyra/ gyrb/ rrs/ eis)
    • Absence of signal for at least one of the wild-type probes indicates resistance of the tested strain to the respective antibiotic
  • Mutation probes
    • Detect most common resistance-mediating mutations (gyra/ gyrb/ rrs/ eis)
    • Each pattern deviating from the wild type pattern indicates resistance

 

Important: For an accurate evaluation, align provided template separately for each locus with the respective Locus Control band.

 

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