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LPA Troubleshooting for No Signal Except Conjugate Control [CC]
Learning Objectives-
There could be overall weak or no signals on the strip, including or except, the Conjugate Control (CC) zone.
Figure: LPA showing No Signal Except CC
Reasons and Troubleshooting for No Signal Except CC are given in the table below:
|
REASON FOR ERROR |
SOLUTION |
|---|---|
|
The quality of extracted DNA does not allow efficient amplification |
Repeat the extraction step |
|
Amplification Mixes (AM-A and AM-B) were either not mixed properly or were added in the wrong amounts |
Prepare a new master mix and repeat the test |
|
The incubation temperature was too high |
Repeat reverse hybridization |
|
The extracted bacterial species cannot be detected by the Universal Control |
Use an alternative identification method |
Resources
- GenoType MTBDR plus ver 2.0 kit, Instructions for Use.
- GenoType MTBDRsl VER 2.0 kit, Instructions for Use.
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