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There could be overall weak or no signals on the strip, including or except, the Conjugate Control (CC) zone.

Figure: LPA showing No Signal Except CC 

 

 

 

Reasons and Troubleshooting for No Signal Except CC are given in the table below:

 

Table: Reasons and Troubleshooting for No Signal Except CC

REASON FOR ERROR

SOLUTION

The quality of extracted DNA does not allow efficient amplification 

Repeat the extraction step​

Amplification Mixes (AM-A and AM-B) were either not mixed properly or were added in the wrong amounts​

Prepare a new master mix and repeat the test​ 

The incubation temperature was too high​

Repeat reverse hybridization​

The extracted bacterial species cannot be detected by the Universal Control

 Use an alternative identification method

 

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