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The daily maintenance of the Bio Safety Cabinet (BSC) should be performed by lab staff and include:

 

  • Surface decontamination of the work zone
  • Cabinet power-up alarm verification
  • Removal of unnecessary items from the BSC
  • Flow check (inflow and downflow velocity)
  • Smoke pattern test
  • Entry in the logbook (shown in Table 2)

 

The weekly maintenance tasks to be performed by lab staff are:

  • Surface decontamination of the drain pan
  • Surface cleaning of fluorescent (FL) and ultraviolet (UV) lamps
  • Front sash cleaning

 

Biosafety cabinets must undergo certification:

  • At the time of installation
  • Whenever they are moved
  • Following any repairs or filter changes

 

The BSCs also require regular (annual) maintenance to ensure proper functioning. 

Delaying maintenance or using underqualified personnel to conduct maintenance can put laboratory workers at risk.

 

Annual Maintenance - To be performed by an external agency

Parameters verified:

  • Down flow and inflow velocities
  • Particle count test
  • Airflow smoke patterns
  • Poly Alpha Olefin (PAO) test for High Efficiency Particulate Air (HEPA) filter integrity

 

The table 1 below summarizes the description of maintenance tasks to be performed and their frequency:

 

Table 1: Maintenance Tasks to be Conducted on the BSC

Description of task to be performed

Maintenance to be carried out every

Day

Week

Month

Quarter

Annually

1. Surface decontaminate the work zone

Yes

 

 

 

 

2. Cabinet power-up alarm verification

Yes

 

 

 

 

3. Decontaminate the work surface and interior surfaces with 5% phenol followed by 70% alcohol

Yes

 

 

 

 

4. Note the manometer’s reading, indicating any fall in pressure flowing through the HEPA filter

Yes

 

 

 

 

5. Thoroughly surface decontaminate the drain pan

 

Yes

 

 

 

6. Check the paper catch for retained materials

 

Yes

 

 

 

5. Clean the exterior surfaces of the cabinet

 

 

Yes

 

 

7. Check all service fixtures for proper operation

 

 

Yes

 

 

8. Inspect the cabinet for any physical abnormalities/ malfunction

 

 

 

Yes

 

9. Re-certification

 

 

 

 

Yes

10. Change UV-lamp

 

 

 

 

8000-9000 hrs. of use

 

Table 2: Maintenance Log Sheet

Date

Time of use

Cumulative duration of use

Cumulative duration of use of UV lamps

Visual alarm

Sound alarm

Smoke test

Airflow

m/s

(for class II BSC)

Observation

Operator’s 

Sign

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Change UV lamps after XXX hours of use (according to manufacturer's recommendations)

 

 

 

 

 

 

 

Resources

 

 

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