Precautions for Using the Biosafety cabinet [BSC]

There are certain Do’s & Don'ts that should be followed while operating the Bio Safety Cabinet (BSC). 

Do’s:

• Read the Standard Operating Procedure (SOP) before starting the work inside the BSC.
• Carry out functionality tests (smoke test, pressure gauge monitoring) before use, and maintain a record at installation and after the maintenance of BSC.
• Check that the sash is at normal operating height (READY state). Adjust the chair height so that your face is above the sash.
• Always use good microbiological practice when working inside the BSC.
• Disinfect work surface, interior walls and interior window surface before and after work.
• Maintain the log sheet after every run of BSC and perform the maintenance activity as required.

Don’ts:

• Never work in the cabinet with the ultraviolet light ON.
• BSC should not be used with the sash window above the design opening mark.
• Perform all the work on or over the work surfaces; avoid working over or placing items on the front grill or blocking the front or rear grills.
• To reduce turbulence, avoid rapid movement while working.
• Avoid unnecessary movement of hands and arms in and out of the cabinet.
• Open flames (Bunsen burners) should never be used inside the BSC; open flames actually create turbulence that disrupts the air flow and damages the filters.
• Never operate the cabinet when any alarms are activated.

Resources

• WHO TB Lab Safety Manual, 2012, p35.

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