CBNAAT Troubleshooting: Error- 5006, 5007, 5008 and 5009 [PCC Failed]

Errors 5006, 5007, 5008 and 5009 (Probe Check Control Failed) sometimes appear on the Cartridge-based Nucleic Acid Amplification Test (CBNAAT) machine in TB laboratories and need troubleshooting.

Problem:

Probe Check Control (PCC) failed, and the test was stopped before amplification.

Origins:

• Incorrect storage of cartridges (probe integrity issues detected)
• Dust on optical filters
• Sample too viscous
• Incorrect sample volume
• Improper fluid transfer (bubbles)

Solutions:

• Store the kits between 2 - 28°C.
• Use an optical brush to clean the optical filters (without a cleaning solution).
• Make sure the sample is totally liquified before transferring it to the cartridges.
• Add the correct volume of the specimen.
• Avoid making bubbles.

Video : CBNAAT Troubleshooting: Error- 5006, 5007, 5008 and 5009 [PCC Failed]

Video : Probe Check Control (PCC)

Resources

• GeneXpert Package Insert