PCR Amplification of the Target genes in Truenat

Truenat Mycobacterium tuberculosis (MTB) and Truenat MTB Rifampicin (Rif) Dx Assays amplify target genes through Polymerase Chain Reaction (PCR).

The presence of mutant gene is detected using probe melt assay.

The target genes include:

• Truenat MTB - ribonucleoside-diphosphate reductase gene nrdZ (the precursor for DNA synthesis) 
• Truenat MTB Rif Dx assay – Rifampicin Resistance Determining Region (RRDR) region of the RNA polymerase Beta (rpoB) gene for resistance to Rif (between codon positions 509 and 533)

Figure: Image showing RRDR of the rpoB gene of M. tuberculosis; the target gene of Truenat MTB Rif Dx assay includes codons 509-533

Resources

• Truenat MTB Pack Insert.
• Truenat MTB Rif Pack Insert.
• Practical Guide to Implementation of TrueNAT Tests for the Detection of TB and Rifampicin Resistance.

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