Essential Tools and Reagents Required for Fluorescence Microscopy Staining

Fluorescence microscopy (FM) is used for TB detection and requires specific tools and reagents which are quality assured.

Following are the tools used for Fluorescence Microscopy (FM) staining:

Reagents used for FM staining are: 0.5% Potassium Permanganate, 1% Acid Alcohol and 0.1% Auramine O

Resources

• Manual for Sputum Smear Fluorescence Microscopy, RNTCP
• Laboratory Diagnosis of Tuberculosis by Sputum Microscopy, GLI Initiative
• Electronic Balance and Clean Up Guidelines

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