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The MGIT tube contains a modified Middlebrook 7H9 broth base, which contains large amounts of dissolved oxygen (Figure below). The headspace of the tube is flushed with a carbon dioxide enriched atmosphere.

 

Components of MGIT Medium Tube

 

  • 7.0 ml of modified 7H9 broth base per 1000 ml of purified water consists of the following active ingredients:
    • Modified Middlebrook 7H9 broth base: 5.9 gm
    • Casein peptone 1.25 gm
  • Fluorescent sensor embedded in silicone on the bottom of the tube
  • Tube is flushed with 10% CO2 at the time of filling
  • Capped with polypropylene screw caps

 

Figure: MGIT 960 Tubes with Media (7 ml)

 

 

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