BACTEC MGIT 960 Growth Supplement

The MGIT 960 Growth Supplement (Figure below) is the enrichment added to the MGIT medium prior to inoculation of specimen. 

It provides substances essential to the growth of mycobacteria, such as:

• Oleic acid, that is utilized by tubercle bacteria and plays an important role in the metabolism of mycobacteria. 
• Albumin, that acts as a protective agent by binding free fatty acids which may be toxic to Mycobacterium species, thereby enhancing their recovery. 
• Dextrose, that is an energy source. 
• Catalase, that destroys toxic peroxides that may be present in the medium. 
• Polyoxyethylene stearate, that enhances the growth of Mycobacterium tuberculosis and assists in providing a uniform inoculum.

Figure: MGIT 960 Growth Supplement

Constituents of MGIT 960 Growth Supplement 

15 ml MGIT 960 Growth Supplement contains:

• Bovine Albumin: 50.0 gm
• Dextrose: 20.0 gm
• Catalase: 0.03 gm
• Oleic Acid: 0.1 gm
• Polyoxyethylene state (POES): 1.1 gm

NOTE: It is a sterile product, do not use if turbid or contaminated.
 

Resources

• MGIT Procedure Manual.

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