Procedure for Culture Specimen Processing: Tissue

The procedure for processing tissue specimens in TB cultures is as follows:

• Add saline or water (2 - 4 ml) and homogenize the tissue in a Biosafety Cabinet (BSC) using sterile equipment:
  • Tissue grinder or homogenizer
  • If these are not available, use a mortar and pestle
  • Small tissue specimens may be placed in a petri dish with 2-4 ml sterile water and torn apart with the help of two sterile needles
• Decontaminate the homogenized specimen with N-acetyl L-cysteine - sodium hydroxide (NALC-NaOH) procedure as in processing sputum. Tissue biopsies collected aseptically do not require decontamination procedures.
• Resuspend the sediment in phosphate buffer.

The processed specimen is used to inoculate the MGIT tubes.

Resources

• MGIT Procedure Manual, Mycobacteria Growth Indicator Tube (MGIT) Culture and Drug Susceptibility Demonstration Projects, FIND Training Manual.

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