Preparation of MGIT Tubes for MGIT TB Cultures

Preparation of MGIT tubes occurs after preparation of PANTA when inoculating and incubating MGIT 960 tubes for TB cultures. The preparation steps are shown below.

• Prepare the MGIT tubes in a clean Biosafety Cabinet (BSC) preferably prior to specimen processing.
• Put an absorbent sheet on the work surface of the BSC and soak it with disinfectant (bactericidal for mycobacteria).
• Before preparation of MGIT tubes:
  • Know the number of samples for batch or per day
  • Label each 7 ml MGIT tube with a specimen number
  • Decontaminate all items to be used like micropipettes, racks, tip boxes and vortex
• Aseptically, add 0.8 ml of PANTA mixture to each MGIT tube.
• Inoculate tubes with specimen within 2 hours of adding PANTA.

Figure: Addition of 0.8 ml of PANTA/ Growth supplement mixture to the MGIT tube using a micropipette

Please click the video below to see the full procedure for inoculating and incubating MGIT tubes for TB cultures:

https://empowerschoolofhealth-my.sharepoint.com/:v:/g/personal/admin_empowerschoolofhealth_org/EXh5-goFTP5PvXwEDDp-r4UBvpE3fW4G4OmBRzP296LfTg?e=keEf8t

Resources

• MGIT Procedure Manual, Mycobacteria Growth Indicator Tube (MGIT) Culture and Drug Susceptibility Demonstration Projects, FIND Training Manual.

Kindly provide your valuable feedback on the page to the link provided HERE