Biosafety Requirements in Line Probe Assay [LPA] Laboratory

DNA extraction for Line Probe Assay (LPA) from clinical specimens can be performed in either a BSL-2 or BSL-3 laboratory, while DNA extraction from mycobacterial cultures must be performed in a BSL-3 laboratory. Only after heat-killing of the organism and DNA isolation can the sample be considered non-infectious and moved to the LPA laboratory.

The subsequent steps, i.e., amplification and post-amplification, only require a BSL-1 laboratory.

Aspects of biosafety in LPA laboratory include:

• Facility Design 
  • Workflow description
  • Rooms required to complete LPA procedures
  • Access to authorized personnel only
  • Electricity and backup power supply
• Laboratory Equipment
  • Equipment operation
  • Equipment maintenance
• Safe Lab Practices
  • Cleaning and disinfecting work areas
  • Use of Personal Protective Equipment (PPE)
  • Disinfection and decontamination
  • Biomedical waste management

Resources

• Tuberculosis Laboratory Biosafety Manual.

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