Equipment Required in Different Rooms in the LPA Lab Facility

The design of the LPA facility includes the availability of different rooms to perform different steps. Each room has a set of specific equipment.

There should be a minimum of 4 separate rooms to carry out procedures. The details of these rooms and equipment are elaborated below:

1. DNA Extraction Room: For DNA extraction from decontaminated samples. Table 1 shows the equipment in the DNA extraction room.

2. Master Mix/ Reagent Preparation Room: For the preparation of reagents required in the master mix for PCR. Table 2 shows the equipment required in this room.

Table 2: Equipment in the Master Mix/Reagent preparation room

PCR HOOD	PICO-FUGE	-20ºC FREEZER

3. DNA Amplification Room: For PCR amplification of DNA. Table 3 shows the equipment in this room.

Table 3: Equipment in the DNA Amplification Room

PCR HOOD	THERMAL CYCLER	REFRIGERATOR

4. Hybridization – detection Room: For hybridization of amplicons with probes and detection of bands. Table 4 shows the equipment required in this room.

Table 4: Equipment in the Hybridization-detection Room

TWINCUBATOR	GT BLOT
REFRIGERATOR	WATER BATH

Resources

• Molecular Detection of Drug-resistant Tuberculosis by Line Probe Assay.
• GLI Training Package on LPA.

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