Contamination Control During DNA Extraction in LPA Lab Settings

Contamination of the Polymerase Chain Reaction (PCR) can occur from previously processed specimens. Hence, it is important to take precautions to avoid contamination.

To ensure contamination control during DNA extraction in a Line Probe Assay (LPA) setting, follow the do's and don’ts elaborated below.

DO's​

• Label tools and instruments “For use in LPA laboratory only”.
• Do specimen preparation on a separate day (earlier) and store extracted DNA at 2-4°C.
• Proceed with reagent preparation and amplification/detection on the following day.​
• Always use Biosafety Cabinet (BSC) for DNA extraction.​
• Always use filtered tips.​
• Change gloves if contaminated by specimens.
• Clean and disinfect the work area before and after use with disinfectant.
• Establish regular (e.g., weekly) and thorough laboratory cleaning protocols for floors, doors, walls.

DON’Ts​

• Instruments used in the LPA laboratory should not be used elsewhere.
• Never take any tools/ instruments used for DNA extraction to Reagent Preparation Area.
• Do not try to put cotton plugs into unfiltered tips.
• Do not use flip-cap tubes, always use screwcap tubes.

Resources

• Molecular Detection of Drug-resistant Tuberculosis by Line Probe Assay.
• GLI Training Package on LPA.

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