Amplification of Extracted DNA in the LPA Lab

In the Line Probe Assay (LPA) labs, subsequent to the preparation of the Polymerase Chain Reaction (PCR) Master mix, DNA amplification is carried out.

The DNA amplification steps remain the same in both First Line - Line Probe Assay (FL-LPA) and Second Line - Line Probe Assay (SL-LPA). In DNA amplification, there is the addition of DNA to the master mix, and the amplification of DNA using PCR machine.

The procedure for amplification is shown below. Play the video below to know more.

Click the video below to see the procedure for PCR amplification.

https://empowerschoolofhealth-my.sharepoint.com/:v:/g/personal/admin_empowerschoolofhealth_org/EcOB4yFVDYBLgmb5o-Ika-gBLgGxHg14_YZAlRTrEbacCw?e=WEmRcs

Resources

• GenoType MTBDR plus ver 2.0 Kit, Instructions for Use.
• GenoType MTBDRsl VER 2.0 Kit, Instructions for Use.
• GLI Training Package on LPA.

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