Contamination Control in the Amplification Room in the LPA Lab

Contamination can happen in the amplification room due to exogenous DNA and spillover from last amplification or contaminants or inhibitors entering the reaction tubes.

Do’s and Don’ts for Contamination Control in the Amplification Room 

Do’s

• Label tools and instruments “For use in Amplification Room Only”​.
• Follow unidirectional work flow​.
• Add DNA extract to PCR tubes in PCR hood.
• Change gloves if they get contaminated with amplicons​.
• Always clean work area properly before and after completion of tasks.
• Establish a regular (e.g., weekly) and thorough laboratory cleaning protocol (floors, doors, walls).

Don’ts

• Tools and instruments used in the amplification room cannot be used elsewhere. ​
• Never take anything from the amplification room to the reagent preparation area​.

Resources

• GenoType MTBDR plus ver 2.0 Kit, Instructions for Use.
• GenoType MTBDRsl VER 2.0 Kit, Instructions for Use.
• GLI Training Package on LPA.

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