ZN Microscopy: Staining Process

ZN Microscopy: Staining Process

Learning Objectives

Describe the Staining Process for ZN Microscopy:

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Content

After air drying and heat-fixing of the smear, it needs to be stained for the identification of MTB during microscopy.

The reagents required for the ZN staining procedure are shown in Figure 1. The steps involved in the staining procedure are shown in Figure 2.

Figure 1: Reagents Required for ZN Staining

Figure 2: Steps involved in ZN staining procedure

For a visual representation of the above-mentioned steps, please click the video below.

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Assessment

Question	Answer 1	Answer 2	Answer 3	Answer 4	Correct answer	Correct explanation	Page id	Part of Pre-test	Part of Post-test
When staining ZN slides, it is important to heat the slides until it begins to boil.	True	False			2	When staining ZN slides, it is important to heat the slides gently until you see vapours, but do not boil.		Yes	Yes

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