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Fluorescent Microscopy: Staining Process
Learning ObjectivesFluorescent Microscopy: Staining Process
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After air drying and heat-fixing of the smear, it needs to be stained.
Fluorescence microscopy (FM) staining should always be carried out in a designated area. The reagents required for the FM staining procedure are shown in Figure 1. The steps involved in the staining procedure are shown in Figure 2.
Figure 1: Reagents Required for FM Staining
Figure 2: Steps involved in FM staining procedure
For a visual representation of the above-mentioned steps, please click the video below:
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Laboratory Diagnosis by Sputum Smear Microscopy, GLI Initiative
Assessment
| Question | Answer 1 | Answer 2 | Answer 3 | Answer 4 | Correct answer | Correct explanation | Page id | Part of Pre-test | Part of Post-test |
| What is the counterstain used in FM staining? | Acid Alcohol | Potassium Permanganate | Phenol | Auramine-O | 2 | In FM staining, Potassium Permanganate is used as the counterstain. | Yes | Yes |
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