{"presentation":{"slides":[{"elements":[{"x":2.178649237472767,"y":4.319654427645788,"width":95.86056644880175,"height":90.71274298056156,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"

The Truenat Assay Technology works on Real-time Polymerase Chain Reaction (PCR).<\/strong><\/span><\/span><\/p>\n\n

The key steps include:<\/span><\/p>\n\n

\n\t
Collection of specimens from a presumptive TB\/diagnosed TB Patient (for testing Rif-resistance).<\/span><\/li>\n\t
Liquefaction and lysis of specimen using the Trueprep AUTO MTB sample pre-treatment pack (20 minutes)<\/span><\/li>\n\t
Extraction and purification of DNA using Trueprep AUTO v2 Universal Cartridge-based sample prep kit and Trueprep AUTO v2 Universal Cartridge-based sample prep device (20 minutes)<\/span><\/li>\n\t
Amplification of extracted DNA by the Truelab Real-time micro-PCR analyzer using freeze dry PCR reagent in microtubes on a Truenat MTB chip (35 minutes)<\/span><\/li>\n\t
DNA from positive test results is tested using Truenat MTB RIF Dx chip as a reflex test (55 minutes)<\/span><\/li>\n<\/ol>\n"},"subContentId":"b481e957-ee4f-4e45-afcf-3db6b93ba836","metadata":{"contentType":"Text","license":"U","title":"Untitled Text"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""}],"slideBackgroundSelector":{}},{"elements":[{"x":13.071895424836601,"y":10.75268817204301,"width":82.78867102396515,"height":86.39308855291577,"action":{"library":"H5P.Image 1.1","params":{"decorative":false,"contentName":"Image","file":{"path":"images\/file-6418081c54831.png","mime":"image\/png","copyright":{"license":"U"},"width":1097,"height":809},"expandImage":"Expand Image","minimizeImage":"Minimize Image"},"subContentId":"224161bc-ba7a-4317-831e-90b79799768e","metadata":{"contentType":"Image","license":"U","title":"Untitled Image"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":2.178649237472767,"y":4.319654427645788,"width":97.82135076252723,"height":39.956803455723545,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"
The process flow from sample to result for Truenat Assay Technology is described in the figure below.<\/span><\/p>\n"},"subContentId":"f4f9c91e-0b31-4547-a168-69837dc86184","metadata":{"contentType":"Text","license":"U","title":"Untitled Text"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":4.357298474945534,"y":79.56989247311827,"width":8.714596949891067,"height":5.183585313174946,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"
Figure A<\/strong><\/p>\n"},"metadata":{"contentType":"Text","license":"U","title":"Untitled Text"},"subContentId":"96eb7dbf-a1cd-472a-ab48-378a8e08d140"},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""}],"slideBackgroundSelector":{}},{"elements":[{"x":14.161220043572985,"y":2.150537634408602,"width":81.69934640522877,"height":96.12903225806451,"action":{"library":"H5P.Image 1.1","params":{"decorative":false,"contentName":"Image","file":{"path":"images\/file-641808941e695.png","mime":"image\/png","copyright":{"license":"U"},"width":1098,"height":646},"expandImage":"Expand Image","minimizeImage":"Minimize Image"},"subContentId":"c2035e7a-9897-4ce5-9626-bb4494818a5f","metadata":{"contentType":"Image","license":"U","title":"Untitled Image"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":4.357298474945534,"y":79.56989247311827,"width":7.625272331154684,"height":5.183585313174946,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"
Figure B<\/strong><\/p>\n"},"metadata":{"contentType":"Text","license":"U","title":"Untitled Text"},"subContentId":"d05f9a92-fc00-4bbe-a4b9-34451d8a2314"},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""}],"slideBackgroundSelector":{}},{"elements":[{"x":10.893246187363834,"y":4.301075268817204,"width":88.01742919389979,"height":94.62365591397848,"action":{"library":"H5P.Image 1.1","params":{"decorative":false,"contentName":"Image","file":{"path":"images\/file-641808c3e8554.png","mime":"image\/png","copyright":{"license":"U"},"width":1126,"height":872},"expandImage":"Expand Image","minimizeImage":"Minimize Image"},"subContentId":"619119b2-33a2-4335-b4c0-71bd93ab1b72","metadata":{"contentType":"Image","license":"U","title":"Untitled Image"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":2.178649237472767,"y":86.02150537634408,"width":8.714596949891067,"height":5.183585313174946,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"
Figure C<\/strong><\/p>\n"},"metadata":{"contentType":"Text","license":"U","title":"Untitled Text"},"subContentId":"e78350e4-5a25-4de6-924d-5e116e701813"},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""}],"slideBackgroundSelector":{}},{"elements":[{"x":14.161220043572985,"y":2.150537634408602,"width":74.07407407407408,"height":84.73118279569891,"action":{"library":"H5P.Image 1.1","params":{"decorative":false,"contentName":"Image","file":{"path":"images\/file-641808f77eeb1.png","mime":"image\/png","copyright":{"license":"U"},"width":1119,"height":642},"expandImage":"Expand Image","minimizeImage":"Minimize Image"},"subContentId":"f1a4094f-947a-4aa7-aa13-ada219c4cd61","metadata":{"contentType":"Image","license":"U","title":"Untitled Image"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":4.357298474945534,"y":89.20088135870395,"width":90.19607843137256,"height":10.799136069114471,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"
Figure (A-D): Process flow from sample to result for Truenat Assay Technology; Source: Practical Guide to Implementation of Truenat\u2122 Tests for the Detection of TB and Rifampicin Resistance.<\/em><\/span><\/p>\n\n
<\/p>\n"},"subContentId":"684ea534-fee6-4dc8-9cd5-4e341bd47ec1","metadata":{"contentType":"Text","license":"U","title":"Untitled Text"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":4.357298474945534,"y":79.91360691144709,"width":11.982570806100219,"height":5.183585313174946,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"
Figure D<\/strong><\/p>\n"},"subContentId":"b0809ae1-646d-482d-bd0e-b959a9263ef2","metadata":{"contentType":"Text","license":"U","title":"Untitled Text"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""}],"slideBackgroundSelector":{}}],"keywordListEnabled":true,"globalBackgroundSelector":{},"keywordListAlwaysShow":false,"keywordListAutoHide":false,"keywordListOpacity":90},"override":{"activeSurface":false,"hideSummarySlide":false,"summarySlideSolutionButton":true,"summarySlideRetryButton":true,"enablePrintButton":true,"social":{"showFacebookShare":false,"facebookShare":{"url":"@currentpageurl","quote":"I scored @score out of @maxScore on a task at @currentpageurl."},"showTwitterShare":false,"twitterShare":{"statement":"I scored @score out of @maxScore on a task at @currentpageurl.","url":"@currentpageurl","hashtags":"h5p, course"},"showGoogleShare":false,"googleShareUrl":"@currentpageurl"}},"l10n":{"slide":"Slide","score":"Score","yourScore":"Your Score","maxScore":"Max Score","total":"Total","totalScore":"Total Score","showSolutions":"Show solutions","retry":"Retry","exportAnswers":"Export text","hideKeywords":"Hide sidebar navigation menu","showKeywords":"Show sidebar navigation menu","fullscreen":"Fullscreen","exitFullscreen":"Exit fullscreen","prevSlide":"Previous slide","nextSlide":"Next slide","currentSlide":"Current slide","lastSlide":"Last slide","solutionModeTitle":"Exit solution mode","solutionModeText":"Solution Mode","summaryMultipleTaskText":"Multiple tasks","scoreMessage":"You achieved:","shareFacebook":"Share on Facebook","shareTwitter":"Share on Twitter","shareGoogle":"Share on Google+","summary":"Summary","solutionsButtonTitle":"Show comments","printTitle":"Print","printIngress":"How would you like to print this presentation?","printAllSlides":"Print all slides","printCurrentSlide":"Print current slide","noTitle":"No title","accessibilitySlideNavigationExplanation":"Use left and right arrow to change slide in that direction whenever canvas is selected.","accessibilityCanvasLabel":"Presentation canvas. Use left and right arrow to move between slides.","accessibilityProgressBarLabel":"Choose slide to display","containsNotCompleted":"@slideName contains not completed interaction","containsCompleted":"@slideName contains completed interaction","slideCount":"Slide @index of @total","containsOnlyCorrect":"@slideName only has correct answers","containsIncorrectAnswers":"@slideName has incorrect answers","shareResult":"Share Result","accessibilityTotalScore":"You got @score of @maxScore points in total","accessibilityEnteredFullscreen":"Entered fullscreen","accessibilityExitedFullscreen":"Exited fullscreen","confirmDialogHeader":"Submit your answers","confirmDialogText":"This will submit your results, do you want to continue?","confirmDialogConfirmText":"Submit and see results","slideshowNavigationLabel":"Slideshow navigation"}}

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STLS: Truenat as a Rapid Molecular Diagnostic Test

Target Audience

Truenat DNA Extraction Cartridge

Overview of the Truenat Testing Process

Inbuilt Controls used in TrueNAT