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Learning Objectives

List methods to identify MGIT growth as MTB that differentiates it from other contaminants

  • Immunochromatographic Test SD Bioline Assay: Participants will acquire knowledge about the SD Bioline assay for rapid identification of MTB.
  • Biochemical Tests for MTB: Participants will gain understanding of various biochemical tests to confirm MTB identification, including:
    • Niacin Production Test: Knowledge about the niacin production test to differentiate MTB from other mycobacteria.
    • Nitrate Reduction Test: Understanding the nitrate reduction test to identify MTB based on its ability to reduce nitrate to nitrite.
    • Catalase Peroxidase Test: Insight into the catalase peroxidase test to assess enzyme activity specific to MTB.
  • SIRE Susceptibility Testing: Participants will learn about SIRE (Streptomycin, Isoniazid, Rifampicin, and Ethambutol) susceptibility testing to determine drug resistance.
  • Pyrazinamide (PZA) Susceptibility Testing: Knowledge about susceptibility testing for Pyrazinamide to assess MTB resistance.
  • Susceptibility of MTB to p-Nitrobenzoic Acid (PNB): Understanding how to test MTB isolates for susceptibility to p-nitrobenzoic acid and interpret the findings.