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Linked Node
Identifying Breaks in Liquid Culture Protocol
Learning ObjectivesIdentify potential deviations and reasons for break in LC protocol and remedial measures
Breaks in Liquid culture protocol can happen due to deviations from standard operating procedures. Some common breaks in the protocol are shown below:
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The Biosafety cabinet is not arranged properly, and the work area is not segregated into clean, dirty and working areas. |
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Using wide mouth beaker for reagent addition |
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Touching the mouth of the beaker with falcon |
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PPE is not worn properly. |
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Inserting the pipette into the falcon can lead to cross-contamination. The PPE is not worn properly. |
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There is a leakage of reagent from the tube. |
Resources
1. Mycobacteriology Laboratory Manual
Assessment
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Question 1 |
Answer 1 |
Answer 2 |
Answer 3 |
Answer 4 |
Correct Answer |
Correct Explanation |
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Part of Pre-Test |
Part of Post-Test |
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The work area in the Biosafety cabinet should be segregated into clean and dirty areas.
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True |
False |
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True |
The Biosafety cabinet work area must be segregated into clean, dirty and working areas.
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Yes |
Yes |
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Question 2 |
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| What are the causes of breaks in LC protocol? | Leakage of reagents | PPE not worn properly | Non-segregation of clean and dirty areas | All | 4 | Deviation from laboratory standard operating procedures leads correctly to a break in protocol. |
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