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The different steps of tube removal are as follows:

1. Visual Observation: ​
 Characteristic flake-like growth (Presumptive Positive) ​
 Uniform Turbidity (Presumptive Contamination) ​


2. Smear: ​
 Corded AFB smear-positive (Culture positive) ​
 Both Acid Fast or Non-Acid Fast Bacilli (Culture with contamination) ​
 Only Non-Acid Fast Bacilli (Contamination)​

3. Inoculate LJ slant/BHI for contamination check by the rate of growth, morphology, smear and immunochromatographic assays

 Growth in 24 – 48 hours (Contamination)

​ No Growth (Contamination free)

​ Cultures should be checked for purity and identification of M. tuberculosis using a rapid immunochromatographic species identification test, such as MPT 64 Ag Assay​ 

Resource

Mycobacteriology Laboratory Manual

Assessment

Question 1

Answer 1

Answer 2

Answer 3

Answer 4

Correct Answer

Correct Explanation

Page id

Part of Pre-Test

Part of Post-Test

AFB smear  should be prepared from a positive MGIT tube.

 

True

False

 

 

True

 

AFB smear  should be prepared from a positive MGIT tube.

 

 

Yes

Yes

Question 2

 

 

 

 

 

 

 

 

 

Sub-culturing a positive MGIT tube on an LJ slant is recommended.

True

False

 

 

True

Sub-culturing a positive MGIT tube on an LJ slant is recommended.

 

Yes

Yes

 

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