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Documentation of MGIT cultures is important to characterise if growth is positive for M. tuberculosis or unexpected result/contamination. The growth is tested for AFB by ZN microscopy and sterility by inoculation on the BHI agar/LJ slant. 

The documentation of growth is as follows:

MGIT Machine Result

Growth Characteristics

ZN Result

BHI agar/LJ slant result

Documentation

Positive

Flakes like growth

Positive

No growth

M. tuberculosis complex detected

Positive

Flakes like growth

Plus turbidity

Positive

Growth

M. tuberculosis complex detected

with contamination

Positive

Flakes like growth

 

Positive

No Growth

M. tuberculosis complex not detected but positive for NTMs

Positive

Turbidity

Negative

Not Applicable

Contamination

Negative

No growth

Not Applicable

Not Applicable

 

Negative for M. tuberculosis complex

Resource

MGIT 960 User manual

Assessment

Question 1

Answer 1

Answer 2

Answer 3

Answer 4

Correct Answer

Correct Explanation

Page id

Part of Pre-Test

Part of Post-Test

Flaky and turbid positive MGIT growth indicates which of the following?

M. tuberculosis complex with contamination

M. tuberculosis complex 

M. tuberculosis complex not detected

NTM detected

1

 

Flaky and turbid positive MGIT growth indicates M. tuberculosis complex with contamination.

 

Yes

Yes

Question 2

 

 

 

 

 

 

 

 

 

Flakes like growth, MGIT positive but does not grow on BHI/LJ indicates which of the following?

 

M. tuberculosis complex with contamination

M. tuberculosis complex 

M. tuberculosis complex not detected but positive for NTMs

E. coli detected

3

Flakes like growth, MGIT positive but does not grow on BHI/LJ indicates M. tuberculosis complex not detected but positive for NTMs.

 

 

Yes

Yes

 

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