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The SD Bioline is a solid-phase immunochromatographic assay that is inexpensive, easy to use and readily available. They are easily stored at room temperature and allow the result interpretation within 15 -20 minutes.

Interpretation of Result: 

 

1) If a purple-red line appears in the Test area [T] and the control area [C], then the culture/strain is positive for the presence of the MPT64 antigens. 

 

2) If a purple-red line appears in the Control area [C] but not in the Test area [T], then the culture/strain is negative for the presence of the MPT64 antigens. 

 

3) If a purple-red line appears only in the Test area [T] and not in the Control area [C], then the test is invalid.

 

Resource

Mycobacteriology Laboratory Manual 

Assessment

Question 1

Answer 1

Answer 2

Answer 3

Answer 4

Correct Answer

Correct Explanation

Page id

Part of Pre-Test

Part of Post-Test

If a purple-red line appears only in the Test area [T] and not in the Control area [C], then the test is invalid.

 

True

False

 

 

True

If a purple-red line appears only in the Test area [T] and not in the Control area [C], then the test is invalid.

 

 

Yes

Yes

Question 2

 

 

 

 

 

 

 

 

 

If a purple-red line appears in the Control area [C] and in the Test area [T], then the culture/strain is negative for the presence of the MPT64 antigens. 

 

True

False

 

 

False

For a culture/strain to be negative for the presence of the MPT64 antigen, a purple-red line should appear in the Control area [C] but not in the Test area [T].

 

Yes

Yes

 

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