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Biochemical Tests for MTB

An experienced laboratory technologist may make the presumptive diagnosis of tuberculosis on the basis of the morphological characteristics of tubercle bacilli, but it is best to do confirmatory tests.

Unfortunately, there is no completely reliable single test that will differentiate M. tuberculosis from other mycobacteria. Nevertheless, the following tests, when used in combination with the characteristics, will enable the precise identification of > 95% of M.tuberculosis strains:

 1) Susceptibility to p-nitrobenzoic acid (PNB)

 2) Niacin test

 3) Catalase activity at 68°C & pH 7. 

Of these, the PNB test can be included along with the drug susceptibility test.

Resource

Revised National TB Control Programme Training Manual for Mycobacterium tuberculosis Culture & Drug susceptibility testing.

Assessment

Question 1

Answer 1

Answer 2

Answer 3

Answer 4

Correct Answer

Correct Explanation

Page id

Part of Pre-Test

Part of Post-Test

Biochemical tests differentiate M. tuberculosis from other mycobacteria.

True

False

 

 

True

PNB, Niacin and Catalase tests are used to differentiate M. tuberculosis from other mycobacteria.

 

Yes

Yes

Question 2

 

 

 

 

 

 

 

 

 

PNB test can be included along with the drug susceptibility test.

True

False

 

 

True

PNB test can be included along with the drug susceptibility test.

 

Yes

Yes

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