Requirements for submission, acceptance and processing for culture Isolates

Equip participants with the skills to manage culture isolates for tuberculosis (TB) diagnosis and drug susceptibility testing (DST).

1. Understand and apply criteria for accepting MTB isolates for DST.
2. Prepare MTB isolates following standard protocols for DST.
3. Execute the preparation of isolates specifically for liquid culture DST.
4. Implement proper storage techniques to maintain the viability of culture isolates.
5. Identify and prepare necessary media and consumables for storing culture isolates.
6. Prepare Selective Middlebrook-7H9 liquid medium and conduct quality control to ensure reliability.
7. Perform accurate inoculation procedures for storing culture isolates.
8. Conduct sterility checks to ensure culture isolates are contamination-free.
9. Properly incubate and store culture isolates to maintain their integrity.
10. Successfully revive frozen cultures while preserving their viability for future testing.