Inoculum Preparation for First and Second-line Phenotypic DST

Inoculum Preparation for First and Second-line Phenotypic DST

Learning Objectives

pre-requisites and steps in inoculum preparation

H5Content

Content

Label the required number of 7 mL MGIT tubes with lab ID. In addition, label tubes with one of each of the following: GC (Growth Control), drug 1, drug 2 etc.
Place the tubes in the suitable AST set carrier (5 tubes or 8 tubes), from left to right: GC, drug 1, drug 2 etc. (as per the number of drugs to be tested).
Aseptically add 800 μl of BACTEC MGIT OADC Supplement to each MGIT tube. Do not use MGIT 960 growth supplement or PZA supplement.
Aseptically pipette 100 μl of the reconstituted drug into the corresponding labelled MGIT tube; e.g., add 100 μl AMK (amikacin) solution to the MGIT tube labelled as “AMK”, etc.
It is important to add the correct drug to the corresponding tube.
Do not add drugs to the MGIT GC tube.

Resource

Mycobacteriology Laboratory Manual

Assessment

Question	Answer 1	Answer 2	Answer 3	Answer 4	Correct answer	Correct explanation	Page id	Part of Pre-test	Part of Post-te
MGIT GC Tube has which of the following?	Dilute test culture suspension	Undiluted test culture suspension	Culture with drugs	Culture without drugs	Answer 1	MGIT GC Tube has Diluted test culture suspension

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