Quality Control of the MGIT DST Inoculum

Quality control of MGIT DST inoculum is essential for quality DST results. The different aspects of inoculum preparation include:

1. Using the right inoculum​​
2. Using pure cultures only​
3. Using fresh cultures​
4. Using slant < 14 days

Quality aspects for Positive MGIT tube ​include:
1. Time period should be plus 1 day to 5 days from the date of positivity​
2. Homogeneous inoculum​
3. Using sterile glass beads and vortex to break up organism clumps​
4. Too high or too low an inoculum may give error results or un-interpretable results
5. Preparing dilutions according to the procedure​
6. Using an accurate pipette to add inoculum to tubes​

Resource

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