{"presentation":{"slides":[{"elements":[{"x":3.2679738562091507,"y":8.657300215982723,"width":93.68191721132898,"height":10.799136069114471,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"

The comparison of the Acid-Fast Bacilli (AFB) and Light Emission Diode (LED) Fluorescence Microscopy is tabulated below:<\/p>\n"},"subContentId":"2939417b-0690-42a9-8ca9-79a5cd2df771","metadata":{"contentType":"Text","license":"U","title":"Untitled Text"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":11.982570806100219,"y":23.758099352051836,"width":37.03703703703704,"height":5.183585313174946,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"

Direct Microscopy for Acid Fast Bacilli (AFB)<\/span><\/span><\/span><\/span><\/span><\/span><\/p>\n"},"subContentId":"a3ab66b3-9d65-40b4-ab41-b716ced54220","metadata":{"contentType":"Text","license":"U","title":"Untitled Text"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":10.893246187363834,"y":79.91360691144709,"width":35.947712418300654,"height":10.799136069114471,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"

Figure 1: Bright Field Microscope used in Ziehl\u2013Neelsen (ZN) staining of AFB<\/span><\/p>\n"},"subContentId":"a3572491-b917-464d-991c-2f64f026a602","metadata":{"contentType":"Text","license":"U","title":"Untitled Text"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":10.893246187363834,"y":30.29762419006479,"width":35.947712418300654,"height":47.30021598272138,"action":{"library":"H5P.Image 1.1","params":{"decorative":false,"contentName":"Image","file":{"path":"images\/file-6435adf54a181.png","mime":"image\/png","copyright":{"license":"U"},"width":471,"height":314}},"subContentId":"d178c93d-8079-486d-9c12-785b23d1cdaa","metadata":{"contentType":"Image","license":"U","title":"Untitled Image"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":53.37690631808279,"y":19.43844492440605,"width":39.21568627450981,"height":10.799136069114471,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"

Light Emission Diode (LED) Fluorescence Microscopy<\/strong><\/span><\/p>\n"},"subContentId":"81019899-20cd-4039-a148-8e4c5b87d8a2","metadata":{"contentType":"Text","license":"U","title":"Untitled Text"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":53.37690631808278,"y":30.32764578833693,"width":32.6797385620915,"height":47.51619870410367,"action":{"library":"H5P.Image 1.1","params":{"decorative":false,"contentName":"Image","file":{"path":"images\/file-6435ae9e64ad4.png","mime":"image\/png","copyright":{"license":"U"},"width":335,"height":291}},"subContentId":"6ea34213-529d-474f-9110-927453be38ef","metadata":{"contentType":"Image","license":"U","title":"Untitled Image"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":53.37690631808278,"y":80.07861771058316,"width":31.59041394335512,"height":12.958963282937365,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"

Figure 2: LED-Fluorescent Microscope used in fluorescent staining of AFB<\/span><\/p>\n"},"subContentId":"2082e72d-46b0-4ad2-b19c-e1a743fa4a9e","metadata":{"contentType":"Text","license":"U","title":"Untitled Text"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""}],"slideBackgroundSelector":{}},{"elements":[{"x":4.357298474945534,"y":6.479481641468682,"width":37.03703703703704,"height":5.183585313174946,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"

Direct Microscopy for Acid Fast Bacilli (AFB)<\/span><\/span><\/span><\/span><\/span><\/span><\/p>\n"},"subContentId":"ad435fa6-3554-4db8-9fdd-f8421150aefd","metadata":{"contentType":"Text","license":"U","title":"Untitled Text"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":1.0893246187363834,"y":12.958963282937365,"width":70.80610021786492,"height":86.39308855291577,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"

\n\t
Method used widely for the diagnosis and confirmation of pulmonary tuberculosis<\/span><\/span><\/span><\/span><\/span><\/li>\n\t
Staining procedure complex Examination at lower magnification is NOT possible<\/span><\/span><\/span><\/span><\/span><\/li>\n\t
Takes more time than LED-FM<\/span><\/span><\/span><\/span><\/span><\/li>\n\t
Low durability<\/span><\/span><\/span><\/span><\/span><\/li>\n\t
Less sensitive<\/span><\/span><\/span><\/span><\/span><\/li>\n\t
As specific as LED-FM<\/span><\/span><\/span><\/span><\/span><\/li>\n\t
No need for an experienced lab technician<\/span><\/span><\/span><\/span><\/span><\/li>\n\t
Technical errors are common<\/span><\/span><\/span><\/span><\/span><\/li>\n\t
Misses the paucibacillary TB cases, especially when the patient is co-infected with HIV<\/span><\/span><\/span><\/span><\/span><\/li>\n<\/ul>\n"},"subContentId":"1afb0b14-2854-44db-8ed2-86fa4980749e","metadata":{"contentType":"Text","license":"U","title":"Untitled Text"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":71.89542483660131,"y":23.758099352051836,"width":23.856209150326798,"height":48.81209503239741,"action":{"library":"H5P.Image 1.1","params":{"decorative":false,"contentName":"Image","file":{"path":"images\/file-6435b06c1449e.png","mime":"image\/png","copyright":{"license":"U"},"width":279,"height":290}},"subContentId":"331db85e-3bc0-40b8-8644-57cd4aec4659","metadata":{"contentType":"Image","license":"U","title":"Untitled Image"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":70.80610021786492,"y":73.43412526997841,"width":22.875816993464053,"height":12.958963282937365,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"
Figure 3: ZN-stained slide used in direct microscopy<\/span><\/p>\n"},"subContentId":"b3a494c0-20d3-42b7-b86e-7748b5ce9803","metadata":{"contentType":"Text","license":"U","title":"Untitled Text"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""}],"slideBackgroundSelector":{}},{"elements":[{"x":4.357298474945534,"y":6.479481641468682,"width":29.411764705882355,"height":6.479481641468682,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"
LED-Fluorescent Microscope <\/strong><\/span><\/p>\n"},"subContentId":"646bd6b7-a5fb-41f4-b915-42479ca21869","metadata":{"contentType":"Text","license":"U","title":"Untitled Text"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":4.357298474945534,"y":10.817127429805616,"width":64.27015250544663,"height":84.23326133909288,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"
\n\t
Method used less commonly for the diagnosis and confirmation of pulmonary tuberculosis<\/span><\/span><\/span><\/span><\/span><\/li>\n\t
Staining procedure simplerExamination at lower magnification is possible<\/span><\/span><\/span><\/span><\/span><\/li>\n\t
Takes 75% less time than ZN and chances to lose scanty slides are also minimized<\/span><\/span><\/span><\/span><\/span><\/li>\n\t
High durability<\/span><\/span><\/span><\/span><\/span><\/li>\n\t
10% more sensitivity than Bright Field ZN microscopy<\/span><\/span><\/span><\/span><\/span><\/li>\n\t
Equally as specific as ZN microscopy <\/span><\/span><\/span><\/span><\/span><\/li>\n\t
Needs an experienced lab technician<\/span><\/span><\/span><\/span><\/span><\/li>\n\t
Technical errors are less common<\/span><\/span><\/span><\/span><\/span><\/li>\n\t
Identifies the paucibacillary TB cases especially when the patient is co-infected with HIV<\/span><\/span><\/span><\/span><\/span><\/li>\n<\/ul>\n"},"subContentId":"8773b472-e214-45d8-95b3-eba54c187614","metadata":{"contentType":"Text","license":"U","title":"Untitled Text"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":71.89542483660131,"y":19.43844492440605,"width":23.965141612200437,"height":48.59611231101512,"action":{"library":"H5P.Image 1.1","params":{"decorative":false,"contentName":"Image","file":{"path":"images\/file-6435b0a890f60.png","mime":"image\/png","copyright":{"license":"U"},"width":310,"height":318}},"subContentId":"9934e154-2aca-4a9f-9f34-eba5e636d885","metadata":{"contentType":"Image","license":"U","title":"Untitled Image"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":71.89542483660131,"y":69.11447084233261,"width":23.965141612200437,"height":15.11879049676026,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"
Figure 4: Auramine-O-stained slide used in fluorescent microscopy<\/span><\/p>\n"},"subContentId":"b676f6e9-7909-4c0a-98eb-4ffac2e01bbe","metadata":{"contentType":"Text","license":"U","title":"Untitled Text"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""}],"slideBackgroundSelector":{}}],"keywordListEnabled":true,"globalBackgroundSelector":{},"keywordListAlwaysShow":false,"keywordListAutoHide":false,"keywordListOpacity":90},"override":{"activeSurface":false,"hideSummarySlide":false,"summarySlideSolutionButton":true,"summarySlideRetryButton":true,"enablePrintButton":false,"social":{"showFacebookShare":false,"facebookShare":{"url":"@currentpageurl","quote":"I scored @score out of @maxScore on a task at @currentpageurl."},"showTwitterShare":false,"twitterShare":{"statement":"I scored @score out of @maxScore on a task at @currentpageurl.","url":"@currentpageurl","hashtags":"h5p, course"},"showGoogleShare":false,"googleShareUrl":"@currentpageurl"}},"l10n":{"slide":"Slide","score":"Score","yourScore":"Your Score","maxScore":"Max Score","total":"Total","totalScore":"Total Score","showSolutions":"Show solutions","retry":"Retry","exportAnswers":"Export text","hideKeywords":"Hide sidebar navigation menu","showKeywords":"Show sidebar navigation menu","fullscreen":"Fullscreen","exitFullscreen":"Exit fullscreen","prevSlide":"Previous slide","nextSlide":"Next slide","currentSlide":"Current slide","lastSlide":"Last slide","solutionModeTitle":"Exit solution mode","solutionModeText":"Solution Mode","summaryMultipleTaskText":"Multiple tasks","scoreMessage":"You achieved:","shareFacebook":"Share on Facebook","shareTwitter":"Share on Twitter","shareGoogle":"Share on Google+","summary":"Summary","solutionsButtonTitle":"Show comments","printTitle":"Print","printIngress":"How would you like to print this presentation?","printAllSlides":"Print all slides","printCurrentSlide":"Print current slide","noTitle":"No title","accessibilitySlideNavigationExplanation":"Use left and right arrow to change slide in that direction whenever canvas is selected.","accessibilityCanvasLabel":"Presentation canvas. Use left and right arrow to move between slides.","accessibilityProgressBarLabel":"Choose slide to display","containsNotCompleted":"@slideName contains not completed interaction","containsCompleted":"@slideName contains completed interaction","slideCount":"Slide @index of @total","containsOnlyCorrect":"@slideName only has correct answers","containsIncorrectAnswers":"@slideName has incorrect answers","shareResult":"Share Result","accessibilityTotalScore":"You got @score of @maxScore points in total","accessibilityEnteredFullscreen":"Entered fullscreen","accessibilityExitedFullscreen":"Exited fullscreen","confirmDialogHeader":"Submit your answers","confirmDialogText":"This will submit your results, do you want to continue?","confirmDialogConfirmText":"Submit and see results","slideshowNavigationLabel":"Slideshow navigation"}}

Search

CDST_LT: Basics of microscopy

Target Audience

Comparing AFB and LED-Fluorescence Microscopy

Ideal Infrastructure Required for Microscopy Centers

Microscopy in TB Diagnosis

Parts of a Microscope

Working with a Microscope