{"presentation":{"slides":[{"elements":[{"x":3.2679738562091503,"y":4.319654427645788,"width":93.46405228758171,"height":51.83585313174946,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"

The National TB Elimination Programme (NTEP) has a standard procedure for sputum smear preparation, the steps for which are listed below:<\/p>\n\n

The tools required for smear preparation include a clean work surface, new and clean glass slides, a discard bucket or a foot-operated bin with a plastic liner, bamboo or wooden applicator sticks or sterile wire loop, spirit lamp and a rack for drying smears.<\/p>\n\n

Use new, clean, unscratched glass slides and label the slide with the laboratory serial number. <\/li>\n\t
Prepare the smear in the centre of the slide covering 3 cm X 2 cm<\/li>\n<\/ul>\n\n
The smear is prepared by using either a wooden stick (Figure 1) or a disposable loop (Figure 2).<\/p>\n"},"subContentId":"9160414b-aae9-4ec9-948e-0e04b181cede","metadata":{"contentType":"Text","license":"U","title":"Untitled Text"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":0,"y":58.31533477321815,"width":43.73669242794297,"height":33.26133909287257,"action":{"library":"H5P.Image 1.1","params":{"decorative":false,"contentName":"Image","file":{"path":"images\/file-6509807455bc8.png","mime":"image\/png","copyright":{"license":"U"},"width":400,"height":154,"originalImage":{"path":"images\/file-643289beb7ae1.png#tmp","mime":"image\/png","height":1080,"width":1920}},"expandImage":"Expand Image","minimizeImage":"Minimize Image"},"subContentId":"eb2295e2-4ece-4ec4-9226-9035a1340eca","metadata":{"contentType":"Image","license":"U","title":"Untitled Image"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":2.178649237472767,"y":94.81641468682506,"width":33.769063180827885,"height":5.183585313174946,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"
Figure 1: Wooden stick used in smear preparation<\/p>\n"},"subContentId":"03db1042-9975-45a0-a6be-b1c796f35341","metadata":{"contentType":"Text","license":"U","title":"Untitled Text"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":59.912854030501094,"y":92.87257019438445,"width":33.769063180827885,"height":5.183585313174946,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"
Figure 2: Disposable loop used in smear preparation<\/p>\n"},"metadata":{"contentType":"Text","license":"U","title":"Untitled Text"},"subContentId":"8e404dee-41dd-4056-a58d-9bb48c75c0dc"},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":54.46623093681917,"y":64.79481641468682,"width":43.57298474945534,"height":21.598272138228943,"action":{"library":"H5P.Image 1.1","params":{"decorative":false,"contentName":"Image","file":{"path":"images\/file-650980a4d6951.png","mime":"image\/png","copyright":{"license":"U"},"width":1013,"height":194,"originalImage":{"path":"images\/file-64328cd43b06a.png#tmp","mime":"image\/png","height":1080,"width":1920}},"expandImage":"Expand Image","minimizeImage":"Minimize Image"},"metadata":{"contentType":"Image","license":"U","title":"Untitled Image"},"subContentId":"530b51a8-3a8e-4a6a-94f5-4754f6621e02"},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""}],"slideBackgroundSelector":{}},{"elements":[{"x":4.357298474945534,"y":8.639308855291576,"width":87.14596949891067,"height":84.23326133909288,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"
Steps for Smear Preparation Using a Wooden Stick<\/strong><\/span><\/p>\n\n

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CDST_LT: Sputum smear preparation

Target Audience

Steps involved in Smear Microscopy

Cleaning and Labelling Slide

Making the smear

Air drying and heat fixing

Qualities of a good sputum smear