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Liquid media are more prone to contamination<\/span><\/strong> than solid media. Therefore, it is essential to process specimens with extreme care, adhering very closely to procedures and recommendations.<\/p>\n"},"subContentId":"8106c0af-5f8b-4985-8702-af0a09623ef3","metadata":{"contentType":"Text","license":"U","title":"Untitled Text"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":6.5359477124183005,"y":23.758099352051836,"width":90.41394335511983,"height":47.51619870410367,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"

Sources of Contamination:<\/strong><\/span><\/p>\n\n

\n\t
Improper or under-decontamination of the specimen<\/span><\/li>\n\t
Thick mucoid specimens that are hard to liquefy <\/span><\/li>\n\t
Prolonged<\/span> storage and transportation time of the specimen after collection. In such situations, especially in hot weather, bacteria tend to overgrow and are hard to kill by routine decontamination procedures.<\/span><\/li>\n\t
Use of non-sterile materials such as pipettes, tubes, etc.<\/span><\/li>\n<\/ul>\n"},"subContentId":"2ea81921-f2da-4ff4-b756-9140a400f379","metadata":{"contentType":"Text","license":"U","title":"Untitled Text"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":5.446623093681917,"y":2.159827213822894,"width":8.714596949891067,"height":17.278617710583152,"action":{"library":"H5P.Image 1.1","params":{"decorative":false,"contentName":"Image","file":{"path":"images\/file-658bb8a7da653.png","mime":"image\/png","copyright":{"license":"U"},"width":128,"height":128}},"subContentId":"741644c0-144b-450f-b71a-763f20ffb4ff","metadata":{"contentType":"Image","license":"U","title":"Untitled Image"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":6.535947712418301,"y":73.58401727861772,"width":89.32461873638344,"height":17.278617710583152,"action":{"library":"H5P.Shape 1.0","params":{"shape":{"fillColor":"rgb(255, 224, 196)","borderWidth":"0","borderStyle":"solid","borderColor":"#000","borderRadius":"0"},"type":"rectangle","line":{"borderColor":"#000000","borderWidth":"1","borderStyle":"solid"}},"subContentId":"1a75090c-0ee4-4fd5-aa91-a38852051175","metadata":{"contentType":"Shapes","license":"U"}},"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false},{"x":8.714596949891067,"y":77.7537796976242,"width":82.78867102396515,"height":10.799136069114471,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"
The incidence of contamination with bacteria (other than mycobacteria) varies from laboratory to laboratory, depending upon several factors<\/p>\n"},"metadata":{"contentType":"Text","license":"U","title":"Untitled Text"},"subContentId":"d402463f-7c7d-4db1-8f4e-cd4ba26cc150"},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""}],"slideBackgroundSelector":{}},{"elements":[{"x":5.445108932461873,"y":6.491490280777537,"width":90.41394335511983,"height":36.717062634989205,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"
According to the NTEP guidelines, up to a 5% contamination rate<\/span><\/strong> is acceptable in cultures of clinical specimens on solid media. However, for liquid media, slightly higher contamination may be accepted (up to 7-8%).<\/span><\/strong><\/p>\n\n
A very low contamination rate (less than 3%) <\/span><\/strong>may indicate too harsh a decontamination process, which would also affect the growth of mycobacteria and may reduce the positivity rate and increase the time-to-detection of positive mycobacterial culture.<\/p>\n"},"metadata":{"contentType":"Text","license":"U","title":"Untitled Text"},"subContentId":"7a1aff23-d452-44c7-a1d5-62dff8346886"},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""}],"slideBackgroundSelector":{}}],"keywordListEnabled":true,"globalBackgroundSelector":{},"keywordListAlwaysShow":false,"keywordListAutoHide":false,"keywordListOpacity":90},"override":{"activeSurface":false,"hideSummarySlide":false,"summarySlideSolutionButton":true,"summarySlideRetryButton":true,"enablePrintButton":false,"social":{"showFacebookShare":false,"facebookShare":{"url":"@currentpageurl","quote":"I scored @score out of @maxScore on a task at @currentpageurl."},"showTwitterShare":false,"twitterShare":{"statement":"I scored @score out of @maxScore on a task at @currentpageurl.","url":"@currentpageurl","hashtags":"h5p, course"},"showGoogleShare":false,"googleShareUrl":"@currentpageurl"}},"l10n":{"slide":"Slide","score":"Score","yourScore":"Your Score","maxScore":"Max Score","total":"Total","totalScore":"Total Score","showSolutions":"Show solutions","retry":"Retry","exportAnswers":"Export text","hideKeywords":"Hide sidebar navigation menu","showKeywords":"Show sidebar navigation menu","fullscreen":"Fullscreen","exitFullscreen":"Exit fullscreen","prevSlide":"Previous slide","nextSlide":"Next slide","currentSlide":"Current slide","lastSlide":"Last slide","solutionModeTitle":"Exit solution mode","solutionModeText":"Solution Mode","summaryMultipleTaskText":"Multiple tasks","scoreMessage":"You achieved:","shareFacebook":"Share on Facebook","shareTwitter":"Share on Twitter","shareGoogle":"Share on Google+","summary":"Summary","solutionsButtonTitle":"Show comments","printTitle":"Print","printIngress":"How would you like to print this presentation?","printAllSlides":"Print all slides","printCurrentSlide":"Print current slide","noTitle":"No title","accessibilitySlideNavigationExplanation":"Use left and right arrow to change slide in that direction whenever canvas is selected.","accessibilityCanvasLabel":"Presentation canvas. Use left and right arrow to move between slides.","accessibilityProgressBarLabel":"Choose slide to display","containsNotCompleted":"@slideName contains not completed interaction","containsCompleted":"@slideName contains completed interaction","slideCount":"Slide @index of @total","containsOnlyCorrect":"@slideName only has correct answers","containsIncorrectAnswers":"@slideName has incorrect answers","shareResult":"Share Result","accessibilityTotalScore":"You got @score of @maxScore points in total","accessibilityEnteredFullscreen":"Entered fullscreen","accessibilityExitedFullscreen":"Exited fullscreen","confirmDialogHeader":"Submit your answers","confirmDialogText":"This will submit your results, do you want to continue?","confirmDialogConfirmText":"Submit and see results","slideshowNavigationLabel":"Slideshow navigation"}}

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CDST_LT: Liquid culture contamination

Target Audience

Liquid Culture Contamination and sources of contamination

Monitoring Liquid Culture Contamination