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The learner will be able to discuss the progression of TB from exposure to disease.

Discuss NAAT as a class of TB diagnostic technology and their various types.

Describe in short the laboratory method of assessing drug susceptibility by culturing in presence of antibiotic.

Discuss that culture DST is only performed in select advanced laboratories in the country.

Describe the laboratory method of culturing TB bacteria and its historic utility in diagnosing TB and that it still continues to be the gold standard diagnostic test for TB.

Discuss the solid and liquid culture types along with its major advantages/ disadvantages.

Discuss that Culture laboratories are setup in only advanced laboratories in select locations in the country.

Explain the role of LPA in the diagnosis of DRTB including both FL and SL LPA.

Provide an overview of the SLPA

Indications for use of SLLPA

Results it provides and its interpretations

Describe the FL LPA as a test for diagnosis of INH Resistance and Rifampicin Resistance.

1. Provide an overview of the LPA Test- A rapid molecular test available at centralized laboratories using a combination of PCR, Gel Electrophoresis technologies. Primarily used for the diagnosis of DRTB. 
2. Discuss the important advantages and disadvantages of the platform.
   1. Rapid test 
   2. Multiple tests at a time-Larger throughput
   3. lower sensitivity requiring culture
   4. Many genetic probes tested simultaneously
3. Provide a brief overview(pictures) of the LPA laboratory and processing. 

The learner will be able to list and discuss acceptable tests for TB diagnosis as per algorithms under NTEP.

Provide an overview of the process that results in a TB case diagnosis.

1. Screening population for signs and symptoms for TB
2. TB Diagnostic Testing for pulmonary and extra-pulmonary TB
3. Clinical diagnosis of TB
4. Diagnosis of Drug resistance
5. Referral for diagnosis and sample transportation