Pending Review

Describe examples of banding patterns of test result interpretation for First Line - Line Probe Assay (FL - LPA).

• Understand the nuances and challenges associated with interpreting First Line - Line Probe Assay (FL-LPA) results for predicting drug resistance in special cases.
• Learn about some specific scenarios/special cases.

Understand the significance of zone development or lack thereof in Wild Type (WT) and Mutation (MUT) probes in predicting drug resistance in First Line - Line Probe Assay (FL-LPA).

• Describe the Key Zones of the FL - LPA Evaluation Strip.
• Elaborate key considerations during the evaluation of an LPA strip.

Controlling contamination in LPA lab.

Describe the general instructions for performing Hybridization Using GT BLOT in LPA Lab Settings.

• Describe the test reagent kits Used for First Line (FL) and Second Line (SL) Line Probe Assay (LPA).
• Discuss the components of these kits.

• Describe the purpose of DNA extraction.
• Process of extraction of DNA from a clinical specimen and culture isolates (LJ, liquid).

Describe the uses, features and instructions for maintenance of the Twincubator as