CDST_LT-M13: LC-DST

Target Audience

• Sr. LT- CDST

• CDST_LT: LT DST Introduction

  • MTB Drug Resistance
  • Mechanism of action-anti TB drugs first line and second line
  • Drug Susceptibility Methods

• CDST_LT: Requirements for submission, acceptance and processing of isolates for DST

  • Requirements for submission, acceptance and processing for culture Isolates
  • Acceptance of MTB Isolates for DST
  • Preparing the Isolate for DST
  • Preparing the Isolate for Liquid Culture DST

• CDST_LT: SIRE susceptibility testing

  • SIRE Susceptibility Testing
  • Critical Concentrations and Reconstitution of SIRE Drugs

• CDST_LT: Pyrazinamide (PZA) susceptibility testing

  • Pyrazinamide PZA Susceptibility Testing
  • Critical Concentration and Reconstitution of PZA

• CDST_LT: Second line drug testing

  • Second-line Phenotypic Drug Susceptibility Testing
  • Critical Concentration and formulations of Second-line Anti-TB Drugs for DST
  • Preparation and Storage of Second-line Anti-TB Drugs used for DST

• CDST_LT: Inoculum preparation and incubation for MGIT DST

  • Inoculum Preparation for First and Second-line Phenotypic DST
  • Inoculum Preparation from MGIT Positive Culture
  • Inoculation of Growth Control Tube/Drug Containing Tubes
  • PZA Inoculum Preparation
  • Inoculation of PZA Growth Control/Drug Containing Tube
  • Incubation of MGIT 960 DST Sets

• CDST_LT: Reading and reporting of MGIT 960 DST results

  • Principle of MGIT DST Reading
  • Procedure for Reading MGIT DST Results
  • Unloaded MGIT DST Report
  • Validation of Resistant/Unexpected MGIT DST Results
  • LC DST Recording and Reporting
  • Tuberculosis Laboratory Register
  • Drug Susceptibility Testing Logs
  • Final Result Reporting

• CDST_LT: MGIT DST quality control

  • MGIT DST Quality Control
  • Standard Controls
  • Procedure of Quality Control for DST
  • Quality Control of the MGIT DST Inoculum
  • Quality Control in DST results interpretation

• CDST_LT: Quality assurance of TB Culture & DST laboratories

  • Quality Assurance in TB C&DST Laboratories
  • C&DST Laboratory Arrangement and Administration
  • Pre-examination QA Procedures in C&DST Laboratories
  • Examination QA Procedures in C&DST Laboratories
  • Post-examination QA Procedures in C&DST Laboratories
  • Quality Improvement Activities in C&DST Laboratories
  • External Quality Assurance for C&DST laboratories