CDST_LT: LPA procedures

Target Audience

• Sr. LT- CDST

• Specimen Preparation for LC and LPA Labs

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• Extraction of DNA from Smear Positive Clinical Specimen, LJ and Liquid Media in LPA Lab Settings

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• Contamination Control During DNA Extraction in LPA Lab Settings

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• LPA PCR Reagent Preparation: Test Reagents Kit

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• PCR Reagent Preparation Procedure in the LPA Lab

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• Contamination Control During PCR Reagent Preparation in the LPA Lab

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• Amplification of Extracted DNA in the LPA Lab

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• Contamination Control in the Amplification Room in the LPA Lab

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• Overview of Hybridization in Line Probe Assay and Genotypic Drug Sensitivity Testing

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• Hybridization Setup in LPA and Genotypic DST

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• Hybridization Using Twincubator in LPA Lab Settings

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• Hybridization Using GT BLOT in LPA Lab Settings

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• Contamination Control in the Hybridization Area in LPA Lab Settings

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