STLS: Truenat

Target Audience

• STLS

• STLS: Truenat as a Rapid Molecular Diagnostic Test

  • Truenat DNA Extraction Cartridge
  • Overview of the Truenat Testing Process
  • Inbuilt Controls used in TrueNAT 

• STLS: Specimen processing for Truenat

  • Processing of Sputum Sample before Truenat Testing
  • TrueNAT Sample Processing: Other Body Fluids
  • DNA Extraction in TrueNAT MTB and TrueNAT MTB/RIF DX Assay
  • PCR Amplification of the Target genes in Truenat

• Truenat: Testing using Truenat

  • Unloading the Truenat Cartridge
  • Using Truenat autoanalyzer for MTB testing
  • Loading the elute onto the Truenat MTB Chip
  • Amplification and Detection of MTB and MTB-Rif

• STLS: Truenat result Interpretation and recording

  • Truenat MTB Assay Results Interpretation: Visualization
  • TrueNAT MTB Assay Results Interpretation: Detection of Mycobacterium tuberculosis and RIF Resistance
  • Retrieving test results from the Truenat machine
  • Recording Truenat Results
  • Recording and reporting the NAAT results
  • Reset Procedure for Truenat Testing

• STLS: Troubleshooting in Truenat

  • Interfering Substances causing errors in NAAT Assay
  • Monitoring Different Types of Errors with Truenat
  • Trueprep Error Classes and actions
  • Truelab Error Classes and actions
  • Troubleshooting Failures Without Error Codes

• STLS: Truenat Instrument maintenance and record keeping

  • Maintenance of the Truenat Instrument - General Principles
  • Truenat Instrument Installation Criteria
  • Daily, Weekly and Monthly Maintenance of Truenat Instrument
  • NAAT Instrument Monthly Data Archive and Data Back-up
  • Annual Maintenance of the Truenat Instrument