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CDST_LT-M4: Smear microscopy

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  2. CDST_LT-M4: Smear microscopy

Target Audience

  • Sr. LT- CDST
  • CDST_LT: Basics of microscopy

    • Comparing AFB and LED-Fluorescence Microscopy
    • Ideal Infrastructure Required for Microscopy Centers
    • Microscopy in TB Diagnosis
    • Parts of a Microscope
    • Working with a Microscope
  • CDST_LT: Sputum smear preparation

    • Steps involved in Smear Microscopy
    • Cleaning and Labelling Slide
    • Making the smear
    • Air drying and heat fixing
    • Qualities of a good sputum smear
  • CDST_LT: Staining sputum smears

    • ZN Microscopy: Staining Process
    • ZN Microscopy: Properties of a well stained slide
    • Fluorescent Microscopy: Staining Process
    • Fluorescent Microscopy: Properties of a well stained slide
  • CDST_LT: Reading smears and reporting microscopy results

    • Process of Reading a Smear
    • Oil immersion and focusing
    • Reading a ZN stained smear
    • Reading a Fluorescent Stained slide
    • Reporting and Recording results of Smear Microscopy (On paper)
    • Reporting results of Microscopy
    • Generating the DMC Laboratory Register from Nikshay
    • Tuberculosis Laboratory Register
  • CDST_LT: Maintenance of the microscope

    • Cleaning a Microscope
    • Storing a Microscope
    • Common issues with Microscope that require technical support
    • Annual Maintenance [AMC] of a Microscope
  • CDST_LT: Maintaining supplies of lab consumables at the DMC

    • Consumables required at a Microscopy Centre
    • Stock Register at a Microscopy Centre
    • Indenting Supplies at a DMC
    • Preparation of Reagents for ZN Microscopy
    • Preparation of Reagents for FM Microscopy
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This work is licensed under Attribution-NonCommercial 4.0 International