MO-TC: TB Laboratories and Diagnostic technologies in NTEP

Target Audience

• Medical Officer- TC/TU

• MO-TC: Diagnostic Technologies and Lab Network

  • Principles and Methods of Testing for TB diagnosis
  • Microscopy in TB Diagnosis
  • Cartridge Based Nucleic Acid Amplification Test [CBNAAT]
  • TrueNAT MTB and RIF Assay
  • Line Probe Assay [LPA]
  • Solid and Liquid Culture in TB
  • Culture Drug Susceptibility Testing [CDST]
  • Laboratory Hierarchy and Network
  • Intermediate Reference Laboratories [IRL] and their role
  • CDST labs and their role
  • NAAT Labs and their role
  • Functions of a Designated Microscopy Centres [DMC]
  • Sputum Collection centres

• MO-TC: TB Detection centres

  • Qualities of a good sputum smear
  • ZN Microscopy: Properties of a well stained slide
  • Fluorescent Microscopy: Properties of a well stained slide
  • Reporting and Recording results of Smear Microscopy (On paper)
  • Reporting results of Microscopy
  • Tuberculosis Laboratory Register
  • Generating the DMC Laboratory Register from Nikshay
  • Annual Maintenance [AMC] of a Microscope
  • Consumables required at a Microscopy Centre
  • Cartridge Based Nucleic Acid Amplification Test [CBNAAT]
  • The CBNAAT Cartridge
  • Consumables required at CBNAAT Lab
  • CBNAAT Testing Process Overview
  • CBNAAT Results Interpretation
  • Recording and reporting the NAAT results
  • Maintenance of CBNAAT Instrument under NTEP
  • Disposal of Infectious Samples and Used Cartridges in CBNAAT Lab
  • TrueNAT MTB and RIF Assay
  • Consumables Required at a Truenat Lab
  • Overview of the Truenat Testing Process
  • Recording and reporting the NAAT results
  • Biosafety measures required for Truenat
  • Disposal of Infectious Samples and Used Cartridges in TrueNAT Laboratories

• MO-TC: Specimen collection and transportation

  • Sputum Collection Process
  • Spot and early morning sputum sample
  • Steps to Ensure a Good Quality Sputum Sample
  • Educating patient on Sputum collection and dispensing Sputum cup
  • Requesting a Test on Nikshay
  • Storing a sputum sample
  • Triple Layer packaging specification for specimen carriers
  • Modes of Transportation in SCT
  • Requirements for Packaging a biological Specimen
  • SOP for Packaging for SCT
  • Specimen Carriers
  • Labelling Specimen Transport Carriers
  • Cool Chain Requirement for the Transport of TB Diagnostic Specimens
  • Dispatching the sample to a C&DST Lab
  • Patient Turnaround Time from Identification to Treatment Initiation Relative to the Laboratory Technology Used in DR-TB

• MO-TC: Quality Assurance of TB Detection Centre

  • False Positivity and False Negativity in Microscopy
  • Measures for Quality Assurance in Microscopy
  • Quality assurance of Stains and Internal Quality Control
  • Onsite Evaluation[OSE]
  • OSE Feedback and action required