CDST_LT-M12:- Liquid culture

Target Audience

• Sr. LT- CDST

• CDST_LT: Introduction to MGIT 960 system

  • MGIT 960 Instrument: Operation

• CDST_LT: Sample processing reagents preparation

  • Precautions for Sample Processing and Reagent Preparation in TB Culture Labs
  • BACTEC MGIT 960 Growth Supplement
  • Quality Control of MGIT 960 Tubes

• CDST_LT: Specimen processing and inoculation

  • Specimen Processing for TB Cultures
  • Procedure for Culture Specimen Processing: Pulmonary specimens
  • Procedure for Culture Specimen Processing: Extrapulmonary specimens
  • Procedure for Culture Specimen Processing: Pus and other muco-purulent specimens
  • Procedure for Culture Specimen Processing: Gastric Aspirate
  • Procedure for Culture Specimen Processing: Bronchial Lavage
  • Procedure for Culture Specimen Processing: Laryngeal Swab
  • Procedure for Culture Specimen Processing: Tissue
  • Procedure for Culture Specimen Processing: Urine
  • Procedure for Culture Specimen Processing: CSF and other body fluids
  • Steps to be done after Culture Specimen Processing
  • Preparation of PANTA for MGIT TB Cultures
  • Preparation of MGIT Tubes for MGIT TB Cultures
  • Inoculation of MGIT Tubes
  • Incubation of MGIT Tubes

• CDST_LT: Growth detection

  • BACTEC MGIT 960 System Growth Detection
  • Work-up for Positive MGIT Cultures
  • Interpreting MGIT Results

• CDST_LT: Liquid culture contamination

  • Liquid Culture Contamination and sources of contamination
  • Monitoring Liquid Culture Contamination

• CDST_LT: False culture results

  • False Positive MGIT Cultures
  • False Negative MGIT Cultures
  • Impact of False MGIT Culture Results

• CDST_LT: Liquid culture troubleshooting

  • Troubleshooting LC Growth Recovery
  • Troubleshooting LC Detection Time
  • Troubleshooting High Liquid Culture contamination
  • Decontamination of Contaminated MGIT Culture

• CDST_LT: MGIT Reading and reporting

  • Documentation of MGIT Cultures
  • MGIT Culture Reading Schedule
  • MGIT 960 Culture Detection Time Frame
  • MGIT 960 Instrument Reports
  • Reading MGIT 960 Tubes Manually

• CDST_LT: Identification methods for MGIT 960 LC growth

  • LC Identification Methods for MTB/Isolated Mycobacteria
  • MTB growth characteristics and morphology
  • AFB Smears from Liquid Culture
  • Rapid Antigen Detection/Immunochromatographic Assay for MTB
  • Procedure for Immunochromatographic Test SD Bioline Assay/Capillia/TBcID
  • Inoculum Preparation for Immunochromatographic Test SD Bioline Assay/Capillia/TBcID
  • Results interpretation for Immunochromatographic Test SD Bioline Assay/Capillia/TBcID
  • Internal Quality Control Kits for Immunochromatographic Test SD Bioline Assay/Capillia/TBcID
  • Biochemical Tests for MTB
  • Susceptibility of MTB to p-Nitrobenzoic acid (PNB)
  • Niacin production test for MTB
  • Nitrate reduction test for MTB
  • Catalase peroxidase test for MTB

• CDST_LT: Storage of culture isolates

  • Storage of Culture Isolates
  • Media and consumables required to store culture isolates
  • Preparation of Selective Middlebrook- 7H9 Liquid Medium
  • Quality Control of Selective Middlebrook- 7H9 Liquid Medium
  • Inoculation Procedure for Storage of Culture Isolates
  • Sterility Check of Culture Isolates
  • Incubation and Storage of Culture Isolates
  • Revival of the Frozen Cultures