Target Audience
CDST_LT: Molecular basics
- Understanding Key elements of Molecular Biology
- Structure of DNA in a Prokaryotic Cell
- Central Dogma in the Function of DNA: Replication
- Central Dogma in the Function of DNA: Transcription and Translation
- Molecular Basis of Genetic Code and Mutation
- Polymerase Chain Reaction (PCR) Technology for M.TB
- Molecular Mechanism of Multi-drug Resistance in Mycobacterium tuberculosis
CDST_LT: Introduction to LPA
CDST_LT: LPA lab biosafety requirements
- Biosafety Requirements in Line Probe Assay [LPA] Laboratory
- Biosafety Requirements in LPA Facility Design
- Electrical Supply and Back-up Power Requirements in LPA Facilities
- Equipment Required in Different Rooms in the LPA Lab Facility
- LPA Biosafety Essentials: Procedural Biosafety Measures
- LPA Biosafety Essentials: Waste Disposal
CDST_LT: Equipment used in LPA labs
- Biosafety in TB Laboratories
- Precautions for Using the Biosafety cabinet [BSC]
- Biosafety Cabinet [BSC] Class I
- Biosafety Cabinet [BSC] Class II Type A2
- Biosafety Cabinet [BSC] Class III
- Cleaning and Maintenance of the Biosafety cabinet [BSC]
- Biosafety Requirements in the LPA Lab: Refrigerated Centrifuge Use
- How to Use Refrigerated Centrifuges
- Revolutions per minute [RPM] vs Relative centrifugal force [RCF] in the Safety Centrifuge
- Protocol for Cleaning and Maintenance of Refrigerated Centrifuge
- Quality Control Process of the Autoclave
- Maintenance of the Autoclave in TB Labs
- Common Problems and Troubleshooting Related to Autoclaves in TB Lab
- LPA Laboratory Equipment: Micro Pipettes
- LPA Laboratory Equipment: Hot Air Oven
- LPA Laboratory Equipment: Micro Centrifuge
- LPA Laboratory Equipment: Vortex Mixer
- LPA Laboratory Equipment: PCR Hood
- LPA Laboratory Equipment: Thermocycler
- LPA Laboratory Equipment: Water Bath
- LPA Laboratory Equipment: Twincubator
- Laboratory Equipment: Thermometer
CDST_LT: LPA procedures
- Specimen Preparation for LC and LPA Labs
- Extraction of DNA from Smear Positive Clinical Specimen, LJ and Liquid Media in LPA Lab Settings
- Contamination Control During DNA Extraction in LPA Lab Settings
- LPA PCR Reagent Preparation: Test Reagents Kit
- PCR Reagent Preparation Procedure in the LPA Lab
- Contamination Control During PCR Reagent Preparation in the LPA Lab
- Amplification of Extracted DNA in the LPA Lab
- Contamination Control in the Amplification Room in the LPA Lab
- Overview of Hybridization in Line Probe Assay and Genotypic Drug Sensitivity Testing
- Hybridization Setup in LPA and Genotypic DST
- Hybridization Using Twincubator in LPA Lab Settings
- Hybridization Using GT BLOT in LPA Lab Settings
- Contamination Control in the Hybridization Area in LPA Lab Settings
CDST_LT: LPA Recording, interpretation and reporting
- Interpretation of FL-LPA: Examples of Test Results Interpretation
- Evaluation of First-line Line Probe Assay Strip
- Evaluation of Second-line Line Probe Assay Strip
- Interpretation of FL-LPA: Predicting TB Drug-resistance
- Interpretation of FL-LPA: Predicting Drug-resistance in Special Cases
- Interpretation of FL-LPA: Examples of Test Results Interpretation
- Limitations of First-line Line Probe Assay [FL-LPA]
- Reporting First-line Line Probe Assay [FL-LPA] Results
- Reporting the Result of First-line Line Probe Assay [FL-LPA] in Special Cases
- Interpretation of SL-LPA: Predicting Drug Resistance
- Interpretation of SL-LPA: Predicting Drug resistance in Special Cases
- Interpretation of SL-LPA: Interpretation Examples
- Limitations of Second-line Line Probe Assay [SL-LPA]
- Reporting Second Line-Line Probe Assay [SL-LPA] Results
CDST_LT: LPA Troubleshooting and quality assurance
- LPA Troubleshooting for No Signal
- LPA Troubleshooting for No Signal Except Conjugate Control [CC]
- LPA Troubleshooting for Uneven Staining
- LPA Troubleshooting for High Background Colour
- LPA Troubleshooting for Other Possible Reasons
- LPA Quality Assurance [QA] Including Quality Control
- LPA Procedural Quality Control
- LPA Quality Assurance: Proficiency Testing
- LPA Quality Assurance: Cleaning of Lab
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